Ion Exchange Chromatography

Ion exchange chromatography (IEX) separates molecules based on their net charge. Negatively or positively charged functional groups are covalently bound to a solid support matrix yielding a cation or anion exchanger. When a charged molecule is applied to an exchanger of opposite charge it is adsorbed, while neutral ions or ions of the same charge are eluted in the void volume of the column. The binding of the charged molecules is reversible, and adsorbed molecules are commonly eluted with a salt or pH gradient.

Ion exchange media are available in various particle sizes, ionic forms, and purity ranges. Bio-Rad's wide selection of media allows you to choose a support which will provide optimal resolution and efficiency for your application.