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Submerged horizontal gels offer many advantages for nucleic acid separation, and have become a primary tool for the molecular biologist. Agarose gels are easy to cast and readily dissipate heat. These gels allow sample underlaying and prevent electrical field discontinuities caused by wicks or sample well dehydration. Horizontal systems are more economical and easier to use than vertical systems.

The Sub-Cell GT and Sub-Cell Model 96 and 192 systems include many features essential to the variable demands of today's molecular biology laboratories, making casting and running agarose gels simple and efficient. The Sub-Cell systems, known for their rugged sturdiness, have been improved to add the features molecular biologists ask for, including

• UV-transparent gel trays with fluorescent ruler
• Gel-casting gates for casting gels in the electrophoresis cell
• A gel caster that allows tape-free casting of multiple gels and sizes simultaneously
• Easy-to-replace electrode cassettes
• Combs to fit virtually every need
  • Multichannel-pipet compatible (MP)
  • Fixed height drop-in combs for ease in casting gels
  • Adjustable height combs for maximum flexibility in well volume and gel thickness
  • Preparative combs for screening large volumes
• IEC 1010 (EN 61010) safety certification
• Buffer recirculation ports
• Clear plastic construction for easy sample visualization

In addition, high-throughput, restriction-digestion optimized Sub-Cell Models 96 and 192 include

• Combs accommodating up to 48 samples plus three standard lanes per comb with wells spaced to fit multichannel pipets
• Gel trays 25 cm wide in a variety of lengths from 10 to 25 cm

The Sub-Cell GT systems are idea for many applications, including

• Northern and Southern blotting
• Cosmid library restriction analysis
• STS screening
• Microsatellite analysis
• PCR fragment analysis
• RFLP analysis
• DNA fingerprinting

Thirty DNA restriction digest samples electrophoresed in the Wide Mini Sub-Cell GT.

Mini-Sub Cell GT

The speed of DNA separations can be increased using miniature submerged gel electrophoresis. For example, Eco R I or Hind III digests of lambda phage DNA can be easily resolved in 1.5 hours at 60 V using the Mini-Sub Cell GT. Separations of small DNA fragments have been performed in as little as 15 minutes at 150 V. The Mini-Sub Cell GT is about 1/4 the size of the Sub-Cell GT DNA cell, and can resolve up to 30 samples. Its short narrow format allows 7 and 10 cm runs with speed, simplicity, and economy.

Wide Mini-Sub Cell GT

The Wide Mini-Sub Cell GT is suited for multiple sample, rapid screening applications. This popular system has a wide platform that can separate 30 samples per comb. The Wide Mini-Sub Cell GT is the same width as the Sub-Cell GT cell, so the comb holder, combs, and the 15 x 10 cm gel trays are interchangeable with the larger Sub-Cell GT units.

Sub-Cell GT DNA Electrophoresis Cell

The Sub-Cell GT cell is the most versatile submerged horizontal electrophoresis cell, offering the widest choice of gel lengths, sample combs, and separation modes. Up to 30 samples can be resolved over a distance of 25 cm. Using four rows of combs, the cell will run up to 120 analytical samples. Preparative separations of large amounts of sample are accomplished using a preparative comb. Sub-Cell GT systems are ideal in preparation for Southern and northern blotting experiments.

Sub-Cell Model 96 Cell

The Sub-Cell Model 96 electrophoresis cell was developed specifically for multiple sample/high-throughput analysis. The design of the cell and analytical combs is based on the fixed spacing of multichannel pipets used to transfer samples from microplates. Forty-eight nucleic acid samples, plus three DNA size standards, can be run in one row using the 51-well comb. If two combs are used, all 96 samples from a microplate can be electrophoresed simultaneously on one gel. The shorter gel lengths (10 and 15 cm) and 26-well comb also allow the Model 96 cell to be used as the routine apparatus for the molecular biology laboratory. The Model 96 cell contains buffer recirculation ports for applications that require high voltages or extended runs. Buffer recirculation eliminates sample migration problems caused by temperature or pH buffer gradients.

Sub-Cell Model 192 Cell

Like the Model 96 cell, the Sub-Cell Model 192 electrophoresis cell was developed for multiple sample analysis and compatibility with multichannel pipets. However, even higher throughput can be achieved with the Model 192 cell. The Model 192 cell can run gels up to 25 cm long. This gel length allows four or more 51-well analytical combs to be used in order to separate more than two microplates of samples. The longer gels and buffer recirculation ports of the Model 192 cell also make this cell ideal for Southern and northern blotting and separation of cosmid DNA restriction digestions. The 25 x 10 cm and 25 x 15 cm trays, comb holder and combs, and electrode assemblies are interchangeable between the Sub-Cell Model 96 and Model 192 electrophoresis cells.

Original and GT Sub-Cell Systems

All but two of the Sub-Cell GT accessories are compatible with original Sub-Cell systems. The gel trays for the original Mini-Sub cell and Mini-Sub cell GT are incompatible because the new GT Mini-Gel tray is deeper to allow thicker gels. The original Mini-Sub cell tray is still offered. The fixed height drop-in combs will only fit into trays designed for the GT systems. Only these trays have the notches for seating the combs, and with the exception of the mini tray, GT trays can be purchased to fit original Sub-Cell systems. Spare parts for the original and GT Sub-Cell systems are available. Contact your Bio-Rad representative for specific information.