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 Life Science Research > Imaging Instruments > Imaging Instruments by Application > Colorimetric Detection > Molecular Imager VersaDoc MP Imaging Systems
 

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Common Detection Methods

Flat Fielding Technology

Molecular Imager VersaDoc MP Imaging Systems


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Related Information: | Literature |

See Also: | Gel Analysis Software | Blotting |


Cy2.

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Cy5.

Cy2/Cy3/Cy5.
 

SYPRO Ruby protein slide printed using
BioOdyssey Calligrapher miniarrayer.

VersaDoc MP imaging systems are high-quality, flexible instruments that allow you to image a wide range of multiplexed samples, from 1-D and 2-D gels, to chemiluminescent and chemifluorescent blots, to microplates and autoradiograms.

Wide Range of Applications

  • Proteomics and genomics studies
  • 2-D protein gels
  • 1-D nucleic acid and protein gels
  • Western blots
  • Northern and Southern blots
  • Colony counting
  • Microarrays with >400 µm spots
  • Dot and slot blots

The VersaDoc MP offers remarkable quality in craftsmanship with highly uniform F-mount lenses for minimum optical distortion, and exceptionally uniform illumination for accurate quantitation across the entire imaging platen.

Each VersaDoc MP imaging system provides the following:
  • High-energy red, green, and blue excitation for greater sensitivity
  • Application-driven software control
  • Excellent sensitivity and resolution
  • Patented flat fielding technology*
  • Highly uniform data (CV** < 5%)
  • Upgradability
  • Data security — full integration with Quantity One 1-D analysis software and PDQuest 2-D analysis software CFR modules

The VersaDoc MP systems are available in two models:

  • Model MP 4000 — state-of-the-art resolution
  • Model MP 5000 — state-of-the-art sensitivity

VersaDoc MP 4000 System

The VersaDoc MP 4000 is the perfect proteomics imager, offering ultimate flexibility with the ability to image your chemiluminescent western blots, Coomassie Blue-stained 1-D gels, SYPRO Ruby-stained 2-D gels, and multiplex or multifluorescent samples with a single system. The MP 4000 provides excellent resolution for collecting the finest details of every band and spot. The patented flat fielding technology generates highly uniform data for accurate quantitation across small and large 2-D samples.


Image acquired using the VersaDoc 4000.

  • 3.2 megapixel CCD, 53 µm resolution
  • Accurate, quantifiable data with a CV < 5%
  • Molecular weight determination and quantitation of protein samples using Quantity One 1-D analysis software
  • Identification of differences in protein expression using PDQuest 2-D analysis software
  • Data security tools that help you meet US FDA 21 CFR Part 11 regulations (Quantity One and PDQuest CFR modules)

VersaDoc MP 5000 System

The VersaDoc MP 5000 is the ideal luminescent imager. It uses Peltier cooling of a blue-enhanced CCD with high quantum efficiency. Each camera is supercooled to minimize background noise and enhance the signal-to-noise ratio. This high-performance digital camera acquires great images of even faintly luminescent images at speeds faster than film-based detection.


Image acquired using the VersaDoc 5000.

  • Excellent chemiluminescent sensitivity
  • Supercooled to –35°C (absolute) for optimal imaging under low light conditions
  • Maximum limit of detection, with true 16-bit data and a dynamic range that covers 4.8 orders of magnitude
  • Quantitation of differences in sample abundance using Quantity One 1-D analysis software
  • Compliance with data security regulations using Quantity One CFR modules

 
Images of a VersaDoc fluorescent reference plate. Left, VersaDoc image without flat fielding (11% CV); right, VersaDoc imaging system with illumination flat fielding applied (1.1% CV).

* US patent 5,951,838.
** Coefficient of variance.

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