Signal Transduction Assays
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Multiplex Assays for Cellular Signaling Research
The Bio-Plex phosphoprotein detection product line collectively refers to Bio-Plex
phosphoprotein assays and Bio-Plex total target assays. These assays make it possible
to simultaneously monitor multiple phosphorylation events with minimal sample volume
requirements and minimal hands-on time. Whether you're evaluating the phosphorylation
level of specific phosphoproteins or the expression level of specific target proteins,
it's quick and easy to get the data you're looking for. |
Bio-Plex Phosphoprotein Assays
Bio-Plex phosphoprotein assays are bead-based multiplexable assays that directly
detect phosphorylated proteins in lysates derived from cell culture or tissue samples.
Using these 96-well filter-plate format assays, you can profile the specific phosphorylation
state of multiple proteins with as little as 25 µl of lysate. Choose from a broad assay
selection and use the assays individually to analyze samples for one target at a time, or
combine them to create a multiplex assay to test for a specific set of phosphoproteins in
a single sample. Custom-mixed multiplex assays are available through Bio-Rad's online
x-Plex assay service.
Bio-Plex phosphoprotein assays feature:
- Highly specific antibodies exclusively developed and validated by
Cell Signaling Technology, Inc.
- A 96-well filter-plate format that yields significantly
higher throughput than traditional methods
- Higher-specificity sandwich format that
minimizes cross-reaction with other cellular proteins
- Direct measurement of kinases that have been
activated within the cell
- Minimal hands-on time and sample volume requirements; for assay workflow, click here
Bio-Plex Total Target Assays
The selection of Bio-Plex assays includes Bio-Plex total target assays that measure specific
phosphoprotein levels, regardless of phosphorylation state, in cell culture or tissue sample
lysates. While the Bio-Plex total target assay reports the abundance of the target protein in one
well, the Bio-Plex phosphoprotein assay reports the level of phosphorylation of the protein in a
separate well. Bio-Plex total target assays share the same format, protocol, and features of
Bio-Plex phosphoprotein assays.
Correlation of Bio-Plex phosphoprotein and total target assays with western blots. Detection of phosphorylated HSP27 (left) and total HSP27 (right) by Bio-Plex assays (upper panels) and by western blotting (lower panels).
Available Assays
We are committed to the continual introduction of new assays to increase your multiplexing options.
Check in with us often to stay up-to-date on our new releases.
Available Phosphoprotein and Total Target Assays and Their Corresponding Bead Regions*
| Assays |
Phosphoprotein |
Total |
Bead Region |
| Akt Signaling |
| Akt (Ser473) |
 |
 |
75 |
| GSK-3α/β (Ser21/Ser9) |
|
|
18 |
| Cell Cycle/Checkpoint Control |
| p53 (Ser15) |
|
  |
53 |
| p53 (Ser37) |
|
|
53 |
| p53 (Ser46) |
|
|
53 |
| Chromatin Regulation/Acetylation |
| Histone H3 (Ser10) |
|
|
77 |
| Immunology/Inflammation |
| IkappaB-α (Ser32/Ser36) |
|
|
58 |
| NF-kappaB p65 (Ser536) |
|
|
37 |
| STAT2 (Tyr689) |
|
|
32 |
| STAT3 (Ser727) |
|
|
52 |
| STAT3 (Tyr705) |
|
|
52 |
| STAT6 (Tyr641) |
|
|
72 |
| Tyk2 (Tyr1054/Tyr1055) |
|
|
33 |
| Glucose/Energy Metabolism |
| IGF-IR (Tyr1131) |
|
|
43 |
| IR-β (Tyr1146) |
|
|
43 |
| IRS-1 (Ser636/Ser639) |
|
|
76 |
| MAP Kinase Signaling |
| ATF-2 (Thr71) |
|
|
20 |
| c-Jun (Ser63) |
|
|
56 |
| ERK1 (Thr202/Tyr204) |
|
|
38 |
| ERK2 (Thr185/Tyr187) |
|
|
38 |
| ERK1/2 (Thr202/Tyr204, Thr185/Tyr187) |
|
|
38 |
| HSP27 (Ser78) |
|
|
51 |
| JNK (Thr183/Tyr185) |
|
|
34 |
| MEK1 (Ser217/Ser221) |
|
|
40 |
| p38 MAPK (Thr180/Tyr182) |
|
|
36 |
| p90RSK (Thr359/Ser363) |
|
|
35 |
| Neuroscience |
| CREB (Ser133) |
|
|
19 |
| TrkA (Tyr490) |
|
|
73 |
| Translational Control |
| p70 S6 kinase (Thr421/Ser424) |
|
|
55 |
| S6 ribosomal protein (Ser235/Ser236) |
|
|
74 |
| Tyrosine Kinases |
| Bcr-Abl (Tyr245) |
|
|
45 |
| c-Abl (Tyr245) |
|
|
45 |
| c-Abl (Tyr412) |
|
|
45 |
| EGFR (Tyr) |
|
|
20 |
| PDGF receptor-β (Tyr751) |
|
|
57 |
| Src (Tyr416) |
|
|
41 |
|
| * |
EGFR and S6 ribosomal protein assays are not multiplexable; they are available only as singleplex assays. The remaining phosphoprotein assays are multiplexable; however, not all multiplex combinations are recommended. To view a chart showing which multiplexable phosphoprotein assays cannot be multiplexed together, click here. |
How to Order
Bio-Plex phosphoprotein and total target assays and associated reagents are designed to provide up to
96 data points for each assay. Two packaging options are available: one 96-well plate and ten 96-well plates.
For phosphoprotein detection, you will need to order the following components:
Available as Individual, Premixed, or x-Plex Assays
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