MyiQ2 Two-Color Real-Time PCR Detection System

The MyiQ2 system offers two-target analysis capabilities for multiplex PCR and routine detection of single fluorophore experiments using SYBR Green I or other green fluorescent dyes. The system is built on the quality gradient-enabled iCycler thermal cycler and can accurately quantitate targets over a dynamic range of nine orders of magnitude. The iQ5 optical system software, version 2.1, used to control the MyiQ2 system, has advanced features for streamlined gene expression analysis, quantitative assays with multiple standard curves, and other qualitative assays, including allelic discrimination and screening for known mutations.

Key Features

• Multiplexing of up to 2 fluorophores in each reaction well, enabling 2-target detection
• Advanced gene expression analysis by relative quantity (ΔC_T) or normalized expression (ΔΔC_T), accounting for differences in reaction efficiency or use of multiple reference genes
• Gene study feature enables direct comparison of gene expression results from up to 5,000 C_T data points and multiple plates
• Multiple data reporting options to generate publication-quality statistics and graphs, quickly create customizable reports, or export data from tables with 1-step Export to Excel
• User preference settings for managing data files, analysis settings, and access privileges

For more detailed information about the features and functionality of iQ5 optical system software, version 2.1, click here.

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