Real-Time PCR Detection Systems
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PCR Reagents
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PCR Plastic Consumables
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PCR Authorization Statements
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Each of Bio-Rad’s real-time PCR detection systems is an upgrade that adds real-time PCR capabilities to a base thermal cycler. The detection systems incorporate optical modules that enable detection of target sequences labeled with fluorescent probes. Sample quantitation by real-time PCR increases reliability of gene expression analyses, improves viral load assessment, and accelerates throughput in screening applications. Bio-Rad's real-time PCR detection systems range from economical upgrades that allow two-target detection to sophisticated five-target detection systems. The table below lists comparative specifications for all Bio-Rad real-time systems.
Real-Time PCR Detection Systems
MiniOpticon |
MyiQ2 |
CFX96 |
CFX384 |
| Excitation range |
470–500 nm |
470–545 nm |
450–684 nm |
450–650 nm |
| Light source |
Light-emitting diode (LED) array
(48 blue-green LEDs) |
Tungsten-halogen lamp |
6 LEDs in optics shuttle |
5 LEDs in optics shuttle |
| Optical detection |
2 photodiodes |
12-bit CCD camera |
6 photodiodes |
5 photodiodes |
| Detection range |
523–700 nm |
515–585 nm |
515–730 nm |
515–690 nm |
| Multiplex capability |
Up to 2 targets |
Up to 2 targets |
Up to 5 targets |
Up to 4 targets |
| Gradient range |
35–99°C |
40–100°C |
30–100°C |
30–100°C |
| Maximum gradient span |
16°C |
25°C |
24°C |
24°C |
| Maximum ramp rate |
2.5°C/sec |
3.3°C/sec |
5°C/sec |
2.5°C/sec |
| Dimensions (W x D x H) |
18 x 32 x 33 cm |
29 x 58 x 39 cm |
33 x 46 x 36 cm |
33 x 46 x 36 cm |
| Weight |
6.8 kg |
17.6 kg |
21.4 kg |
21.4 kg |
| Base thermal cycler |
MJ Mini |
iCycler |
C1000 |
C1000 |
PCR Legal and Authorization Notices
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